Disinfectants are characterized as chemical liquids that kill or inhibit bacteria. They are used on everyday surfaces in order to lower potential infections of humans and cattle. Here, we seek to identify disinfectants and their respective concentrations that inhibit growth of several relatives of ESKAPE bacteria (Acinetobacter baylyi, Enterobacter aerogenes, Escherichia coli, Pseudomonas putida), Bacillus subtilis, and an unknown bacterium. We hypothesized that applying a disinfectant will result in inhibition of growth of the microorganisms. We harvested isolated bacteria, performed 10-fold serial dilutions of the microorganisms, and measured growth after overnight incubation on agar containing different disinfectants. Our results showed that the application of 50% antibacterial soap used in Spelman College restrooms inhibited bacterial  growth, with the exception of Pseudomonas putida. Neither 50% bleach nor 20% sodium chloride (NaCl) were able to inhibit bacterial growth. The 50% antibacterial soap contains 0.5% Triclosan, an antibacterial and antifungal agent present in certain cleaning products. It is possible that bleach and NaCl require a higher concentration to kill or inhibit bacteria. This research can help find proper concentrations of disinfectants to inhibit bacterial growth in common locations like health buildings, workplaces, schools, and homes.
